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Evaluation of barrier creams against sulphur mustard: (II) in vivo and in vitro studies using the domestic white pig. / Chilcott, Robert; Dalton, Christopher H.; Ashley, Zoe; Allen, Ceri E.; Bradley, Simon T.; Maidment, Michael P.; Jenner, John; Brown, Roger F. R.; Gwyther, Robert J.; Rice, Paul.

In: Cutaneous and Ocular Toxicology, Vol. 26, No. 3, 2007, p. 235-247.

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Harvard

Chilcott, R, Dalton, CH, Ashley, Z, Allen, CE, Bradley, ST, Maidment, MP, Jenner, J, Brown, RFR, Gwyther, RJ & Rice, P 2007, 'Evaluation of barrier creams against sulphur mustard: (II) in vivo and in vitro studies using the domestic white pig', Cutaneous and Ocular Toxicology, vol. 26, no. 3, pp. 235-247. https://doi.org/10.1080/15569520701212373

APA

Chilcott, R., Dalton, C. H., Ashley, Z., Allen, C. E., Bradley, S. T., Maidment, M. P., Jenner, J., Brown, R. F. R., Gwyther, R. J., & Rice, P. (2007). Evaluation of barrier creams against sulphur mustard: (II) in vivo and in vitro studies using the domestic white pig. Cutaneous and Ocular Toxicology, 26(3), 235-247. https://doi.org/10.1080/15569520701212373

Vancouver

Author

Chilcott, Robert ; Dalton, Christopher H. ; Ashley, Zoe ; Allen, Ceri E. ; Bradley, Simon T. ; Maidment, Michael P. ; Jenner, John ; Brown, Roger F. R. ; Gwyther, Robert J. ; Rice, Paul. / Evaluation of barrier creams against sulphur mustard: (II) in vivo and in vitro studies using the domestic white pig. In: Cutaneous and Ocular Toxicology. 2007 ; Vol. 26, No. 3. pp. 235-247.

Bibtex

@article{9821e78aa60e47ad82522dced65d95af,
title = "Evaluation of barrier creams against sulphur mustard: (II) in vivo and in vitro studies using the domestic white pig",
abstract = "Previous studies in our laboratory have demonstrated that barrier creams, comprising perfluorinated polymers, are effective against the chemical warfare agent sulphur mustard (SM) when evaluated using human skin in vitro. The purpose of this follow-up study was to further evaluate three candidate ( perfluorinated) barrier creams against SM ( vapour) using the domestic white pig. The severity and progression of the resulting skin lesions were quantified daily for three weeks post-exposure using biophysical measurements of transepidermal water loss (TEWL) and skin reflectance spectroscopy (SRS). Skin biopsies obtained post-mortem were evaluated by light microscopy and additional skin samples were obtained from adjacent ( unexposed) skin sites for a comparative in vitro skin absorption study. Samples of SM vapour within the dosing chambers were measured ex vivo to ascertain the exposure dose (Ct). The three creams were highly effective against SM in vivo (Ct similar to 5000 mg center dot min center dot m(-3)): After 3 weeks, barrier cream pre-treated sites were not significantly different from control ( unexposed) skin when evaluated by TEWL, SRS or histology. In contrast, skin exposed to SM without pre-treatment showed evidence of persistent damage that was consistent with the slow healing time observed in humans. The amount of SM absorbed in vitro in untreated pig skin was similar to that required to cause comparable lesions in human skin (8 - 20 and 4 - 10 mu g center dot cm(-2), respectively), further validating the use of pigs as a toxicologically-relevant dermal model for SM exposure.",
keywords = "chemical warfare agent, reflectance spectroscopy, skin protection, TEWL, topical skin protectant, TRANSEPIDERMAL WATER-LOSS, PERCUTANEOUS-ABSORPTION, VESICANT BURNS, SKIN, MANAGEMENT",
author = "Robert Chilcott and Dalton, {Christopher H.} and Zoe Ashley and Allen, {Ceri E.} and Bradley, {Simon T.} and Maidment, {Michael P.} and John Jenner and Brown, {Roger F. R.} and Gwyther, {Robert J.} and Paul Rice",
year = "2007",
doi = "10.1080/15569520701212373",
language = "English",
volume = "26",
pages = "235--247",
journal = "Cutaneous and Ocular Toxicology",
issn = "1556-9527",
publisher = "Informa Healthcare",
number = "3",

}

RIS

TY - JOUR

T1 - Evaluation of barrier creams against sulphur mustard: (II) in vivo and in vitro studies using the domestic white pig

AU - Chilcott, Robert

AU - Dalton, Christopher H.

AU - Ashley, Zoe

AU - Allen, Ceri E.

AU - Bradley, Simon T.

AU - Maidment, Michael P.

AU - Jenner, John

AU - Brown, Roger F. R.

AU - Gwyther, Robert J.

AU - Rice, Paul

PY - 2007

Y1 - 2007

N2 - Previous studies in our laboratory have demonstrated that barrier creams, comprising perfluorinated polymers, are effective against the chemical warfare agent sulphur mustard (SM) when evaluated using human skin in vitro. The purpose of this follow-up study was to further evaluate three candidate ( perfluorinated) barrier creams against SM ( vapour) using the domestic white pig. The severity and progression of the resulting skin lesions were quantified daily for three weeks post-exposure using biophysical measurements of transepidermal water loss (TEWL) and skin reflectance spectroscopy (SRS). Skin biopsies obtained post-mortem were evaluated by light microscopy and additional skin samples were obtained from adjacent ( unexposed) skin sites for a comparative in vitro skin absorption study. Samples of SM vapour within the dosing chambers were measured ex vivo to ascertain the exposure dose (Ct). The three creams were highly effective against SM in vivo (Ct similar to 5000 mg center dot min center dot m(-3)): After 3 weeks, barrier cream pre-treated sites were not significantly different from control ( unexposed) skin when evaluated by TEWL, SRS or histology. In contrast, skin exposed to SM without pre-treatment showed evidence of persistent damage that was consistent with the slow healing time observed in humans. The amount of SM absorbed in vitro in untreated pig skin was similar to that required to cause comparable lesions in human skin (8 - 20 and 4 - 10 mu g center dot cm(-2), respectively), further validating the use of pigs as a toxicologically-relevant dermal model for SM exposure.

AB - Previous studies in our laboratory have demonstrated that barrier creams, comprising perfluorinated polymers, are effective against the chemical warfare agent sulphur mustard (SM) when evaluated using human skin in vitro. The purpose of this follow-up study was to further evaluate three candidate ( perfluorinated) barrier creams against SM ( vapour) using the domestic white pig. The severity and progression of the resulting skin lesions were quantified daily for three weeks post-exposure using biophysical measurements of transepidermal water loss (TEWL) and skin reflectance spectroscopy (SRS). Skin biopsies obtained post-mortem were evaluated by light microscopy and additional skin samples were obtained from adjacent ( unexposed) skin sites for a comparative in vitro skin absorption study. Samples of SM vapour within the dosing chambers were measured ex vivo to ascertain the exposure dose (Ct). The three creams were highly effective against SM in vivo (Ct similar to 5000 mg center dot min center dot m(-3)): After 3 weeks, barrier cream pre-treated sites were not significantly different from control ( unexposed) skin when evaluated by TEWL, SRS or histology. In contrast, skin exposed to SM without pre-treatment showed evidence of persistent damage that was consistent with the slow healing time observed in humans. The amount of SM absorbed in vitro in untreated pig skin was similar to that required to cause comparable lesions in human skin (8 - 20 and 4 - 10 mu g center dot cm(-2), respectively), further validating the use of pigs as a toxicologically-relevant dermal model for SM exposure.

KW - chemical warfare agent

KW - reflectance spectroscopy

KW - skin protection

KW - TEWL

KW - topical skin protectant

KW - TRANSEPIDERMAL WATER-LOSS

KW - PERCUTANEOUS-ABSORPTION

KW - VESICANT BURNS

KW - SKIN

KW - MANAGEMENT

U2 - 10.1080/15569520701212373

DO - 10.1080/15569520701212373

M3 - Article

VL - 26

SP - 235

EP - 247

JO - Cutaneous and Ocular Toxicology

JF - Cutaneous and Ocular Toxicology

SN - 1556-9527

IS - 3

ER -