University of Hertfordshire

Fungal diversity and metabolomic profiles in GM and isogenic non-GM maize cultivars from Brazil

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Fungal diversity and metabolomic profiles in GM and isogenic non-GM maize cultivars from Brazil. / Marcon Gasperini, Alessandra; Garcia-Cela, Esther; Sulyok, Michael; Medina, A.; Magan, Naresh.

In: Mycotoxin Research, Vol. 37, No. 1, 01.02.2021, p. 39 - 48.

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Marcon Gasperini, Alessandra ; Garcia-Cela, Esther ; Sulyok, Michael ; Medina, A. ; Magan, Naresh. / Fungal diversity and metabolomic profiles in GM and isogenic non-GM maize cultivars from Brazil. In: Mycotoxin Research. 2021 ; Vol. 37, No. 1. pp. 39 - 48.

Bibtex

@article{6395e5ae01424fd9be5f3ad6a08e5155,
title = "Fungal diversity and metabolomic profiles in GM and isogenic non-GM maize cultivars from Brazil",
abstract = "There is little knowledge of the microbial diversity, mycotoxins and associated secondary metabolites in GM maize and isogenic non-GM cultivars (cvs). This study has quantified the microbial populations and dominant fungal genera in 6 cvs of each type representative of herbicide, pesticide or stacked resistance to both. The predominant mycotoxins and targeted metabolomics profiles were also compared between the two sets of cvs. This showed that the overall fungal populations were 8.8 CFUs g−1 maize. The dominant genera, isolated from maize samples, whether surface-sterilised or not, in all maize cvs were Fusarium, followed by Penicillium, Aspergillus and occasionally Cladosporium and Alternaria. The analysis of the targeted metabolomics showed that approx. 29 different metabolites were detected. These were dominated by fumonisins and minor Penicillium spp. metabolites (questiomycin A and rugulovasine A). Interestingly, the range and number of mycotoxins present in the GM cvs were significantly lower than in the non-GM maize samples. This suggests that while the fungal diversity of the two types of maize appeared to be very similar, the major contaminant mycotoxins and range of toxic secondary metabolites were much lower in the GM cvs.",
keywords = "Aflatoxin, maize, metabolomics, Metabolomics, Maize cultivars, Selective media, GM, Toxic secondary metabolites, Mycotoxigenic fungi, Non-GM, Fungal diversity",
author = "{Marcon Gasperini}, Alessandra and Esther Garcia-Cela and Michael Sulyok and A. Medina and Naresh Magan",
note = "The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, http://creativecommons.org/licenses/by/4.0/.",
year = "2021",
month = feb,
day = "1",
doi = "10.1007/s12550-020-00414-8",
language = "English",
volume = "37",
pages = "39 -- 48",
journal = "Mycotoxin Research",
issn = "0178-7888",
publisher = "Springer-Verlag GmbH and Co. KG",
number = "1",

}

RIS

TY - JOUR

T1 - Fungal diversity and metabolomic profiles in GM and isogenic non-GM maize cultivars from Brazil

AU - Marcon Gasperini, Alessandra

AU - Garcia-Cela, Esther

AU - Sulyok, Michael

AU - Medina, A.

AU - Magan, Naresh

N1 - The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, http://creativecommons.org/licenses/by/4.0/.

PY - 2021/2/1

Y1 - 2021/2/1

N2 - There is little knowledge of the microbial diversity, mycotoxins and associated secondary metabolites in GM maize and isogenic non-GM cultivars (cvs). This study has quantified the microbial populations and dominant fungal genera in 6 cvs of each type representative of herbicide, pesticide or stacked resistance to both. The predominant mycotoxins and targeted metabolomics profiles were also compared between the two sets of cvs. This showed that the overall fungal populations were 8.8 CFUs g−1 maize. The dominant genera, isolated from maize samples, whether surface-sterilised or not, in all maize cvs were Fusarium, followed by Penicillium, Aspergillus and occasionally Cladosporium and Alternaria. The analysis of the targeted metabolomics showed that approx. 29 different metabolites were detected. These were dominated by fumonisins and minor Penicillium spp. metabolites (questiomycin A and rugulovasine A). Interestingly, the range and number of mycotoxins present in the GM cvs were significantly lower than in the non-GM maize samples. This suggests that while the fungal diversity of the two types of maize appeared to be very similar, the major contaminant mycotoxins and range of toxic secondary metabolites were much lower in the GM cvs.

AB - There is little knowledge of the microbial diversity, mycotoxins and associated secondary metabolites in GM maize and isogenic non-GM cultivars (cvs). This study has quantified the microbial populations and dominant fungal genera in 6 cvs of each type representative of herbicide, pesticide or stacked resistance to both. The predominant mycotoxins and targeted metabolomics profiles were also compared between the two sets of cvs. This showed that the overall fungal populations were 8.8 CFUs g−1 maize. The dominant genera, isolated from maize samples, whether surface-sterilised or not, in all maize cvs were Fusarium, followed by Penicillium, Aspergillus and occasionally Cladosporium and Alternaria. The analysis of the targeted metabolomics showed that approx. 29 different metabolites were detected. These were dominated by fumonisins and minor Penicillium spp. metabolites (questiomycin A and rugulovasine A). Interestingly, the range and number of mycotoxins present in the GM cvs were significantly lower than in the non-GM maize samples. This suggests that while the fungal diversity of the two types of maize appeared to be very similar, the major contaminant mycotoxins and range of toxic secondary metabolites were much lower in the GM cvs.

KW - Aflatoxin

KW - maize

KW - metabolomics

KW - Metabolomics

KW - Maize cultivars

KW - Selective media

KW - GM

KW - Toxic secondary metabolites

KW - Mycotoxigenic fungi

KW - Non-GM

KW - Fungal diversity

UR - http://www.scopus.com/inward/record.url?scp=85092504621&partnerID=8YFLogxK

U2 - 10.1007/s12550-020-00414-8

DO - 10.1007/s12550-020-00414-8

M3 - Article

VL - 37

SP - 39

EP - 48

JO - Mycotoxin Research

JF - Mycotoxin Research

SN - 0178-7888

IS - 1

ER -