University of Hertfordshire

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Identification of an inhibitor of transcription in extracts prepared from wheat shoot nuclei

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Identification of an inhibitor of transcription in extracts prepared from wheat shoot nuclei. / Henfrey, R.D.; Slater, R.J.; Proudfoot, L.M.F.; Covey, S.N.

In: Plant Science, Vol. 64, No. 1, 01.01.1989, p. 91-98.

Research output: Contribution to journalArticlepeer-review

Harvard

Henfrey, RD, Slater, RJ, Proudfoot, LMF & Covey, SN 1989, 'Identification of an inhibitor of transcription in extracts prepared from wheat shoot nuclei', Plant Science, vol. 64, no. 1, pp. 91-98.

APA

Henfrey, R. D., Slater, R. J., Proudfoot, L. M. F., & Covey, S. N. (1989). Identification of an inhibitor of transcription in extracts prepared from wheat shoot nuclei. Plant Science, 64(1), 91-98.

Vancouver

Henfrey RD, Slater RJ, Proudfoot LMF, Covey SN. Identification of an inhibitor of transcription in extracts prepared from wheat shoot nuclei. Plant Science. 1989 Jan 1;64(1):91-98.

Author

Henfrey, R.D. ; Slater, R.J. ; Proudfoot, L.M.F. ; Covey, S.N. / Identification of an inhibitor of transcription in extracts prepared from wheat shoot nuclei. In: Plant Science. 1989 ; Vol. 64, No. 1. pp. 91-98.

Bibtex

@article{5f2d92cf969846fda6dfc2aaae6a3e55,
title = "Identification of an inhibitor of transcription in extracts prepared from wheat shoot nuclei",
abstract = "The analysis of run-off transcripts initiated in vitro from the CaMV 35S promoter in a HeLa whole-cell extract was used to assay plant nuclei extracts for the presence of an inhibitor of transcription. Extracts of isolated wheat shoot nuclei contained an activity that inhibited transcription directed from the CaMV 35S promoter. The amount of inhibition was variable between each preparation of the wheat nuclei extract, and was labile to cold storage and heat denaturation. No inhibitory activity could be detected, using the same extraction procedure and assay conditions, in extracts prepared from turnip leaf nuclei. HeLa whole-cell extracts inhibit non-specific transcription in plant extracts",
author = "R.D. Henfrey and R.J. Slater and L.M.F. Proudfoot and S.N. Covey",
year = "1989",
month = jan,
day = "1",
language = "English",
volume = "64",
pages = "91--98",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Identification of an inhibitor of transcription in extracts prepared from wheat shoot nuclei

AU - Henfrey, R.D.

AU - Slater, R.J.

AU - Proudfoot, L.M.F.

AU - Covey, S.N.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The analysis of run-off transcripts initiated in vitro from the CaMV 35S promoter in a HeLa whole-cell extract was used to assay plant nuclei extracts for the presence of an inhibitor of transcription. Extracts of isolated wheat shoot nuclei contained an activity that inhibited transcription directed from the CaMV 35S promoter. The amount of inhibition was variable between each preparation of the wheat nuclei extract, and was labile to cold storage and heat denaturation. No inhibitory activity could be detected, using the same extraction procedure and assay conditions, in extracts prepared from turnip leaf nuclei. HeLa whole-cell extracts inhibit non-specific transcription in plant extracts

AB - The analysis of run-off transcripts initiated in vitro from the CaMV 35S promoter in a HeLa whole-cell extract was used to assay plant nuclei extracts for the presence of an inhibitor of transcription. Extracts of isolated wheat shoot nuclei contained an activity that inhibited transcription directed from the CaMV 35S promoter. The amount of inhibition was variable between each preparation of the wheat nuclei extract, and was labile to cold storage and heat denaturation. No inhibitory activity could be detected, using the same extraction procedure and assay conditions, in extracts prepared from turnip leaf nuclei. HeLa whole-cell extracts inhibit non-specific transcription in plant extracts

UR - http://www.scopus.com/inward/record.url?scp=38249026627&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:38249026627

VL - 64

SP - 91

EP - 98

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 1

ER -