University of Hertfordshire

From the same journal

By the same authors

Standard

In vitro synthesized RNA generated from cDNA clones of both genomic components of Cucurbit yellow stunting disorder virus replicates in cucumber protoplasts. / Owen, Carolyn; Moukarzel, Romy; Huang, Xiao; Kassem, Mona; Eliasco, Eleonora; Aranda, Miguel; Coutts, Robert H.A.; Livieratos, Ioannis.

In: Viruses, Vol. 8, No. 6, doi: 10.3390/v8060170, 14.06.2016, p. E170.

Research output: Contribution to journalArticlepeer-review

Harvard

Owen, C, Moukarzel, R, Huang, X, Kassem, M, Eliasco, E, Aranda, M, Coutts, RHA & Livieratos, I 2016, 'In vitro synthesized RNA generated from cDNA clones of both genomic components of Cucurbit yellow stunting disorder virus replicates in cucumber protoplasts.', Viruses, vol. 8, no. 6, doi: 10.3390/v8060170, pp. E170. https://doi.org/10.3390/v8060170

APA

Vancouver

Author

Owen, Carolyn ; Moukarzel, Romy ; Huang, Xiao ; Kassem, Mona ; Eliasco, Eleonora ; Aranda, Miguel ; Coutts, Robert H.A. ; Livieratos, Ioannis. / In vitro synthesized RNA generated from cDNA clones of both genomic components of Cucurbit yellow stunting disorder virus replicates in cucumber protoplasts. In: Viruses. 2016 ; Vol. 8, No. 6. pp. E170.

Bibtex

@article{d0421d1ef9a5489fb6ae4bc7221ca697,
title = "In vitro synthesized RNA generated from cDNA clones of both genomic components of Cucurbit yellow stunting disorder virus replicates in cucumber protoplasts.",
abstract = "Cucurbit yellow stunting disorder virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a major threat to commercial cucurbit production worldwide. Here, construction of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts able to replicate in cucumber protoplasts. CYSDV RNA1 proved competent for replication; transcription of both polarities of the genomic RNA was detectable 24 h post inoculation. Hybridization of total RNA extracted from transfected protoplasts or from naturally CYSDV-infected cucurbits revealed high-level transcription of the p22 subgenomic RNA species. Replication of CYSDV RNA2 following co-transfection with RNA1 was also observed, with similar transcription kinetics. A CYSDV RNA2 cDNA clone (T3CM8Δ) comprising the 5'- and 3'-UTRs plus the 3'-terminal gene, generated a 2.8 kb RNA able to replicate to high levels in protoplasts in the presence of CYSDV RNA1. The clone T3CM8Δ will facilitate reverse genetics studies of CYSDV gene function and RNA replication determinants.",
keywords = "criniviruses, whitefly-transmitted viruses, Cucurbit yellow stunting disorder virus, infectious clones, protoplasts",
author = "Carolyn Owen and Romy Moukarzel and Xiao Huang and Mona Kassem and Eleonora Eliasco and Miguel Aranda and Coutts, {Robert H.A.} and Ioannis Livieratos",
note = "{\textcopyright} 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).",
year = "2016",
month = jun,
day = "14",
doi = "10.3390/v8060170",
language = "English",
volume = "8",
pages = "E170",
journal = "Viruses",
issn = "1999-4915",
publisher = "MDPI",
number = "6",

}

RIS

TY - JOUR

T1 - In vitro synthesized RNA generated from cDNA clones of both genomic components of Cucurbit yellow stunting disorder virus replicates in cucumber protoplasts.

AU - Owen, Carolyn

AU - Moukarzel, Romy

AU - Huang, Xiao

AU - Kassem, Mona

AU - Eliasco, Eleonora

AU - Aranda, Miguel

AU - Coutts, Robert H.A.

AU - Livieratos, Ioannis

N1 - © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

PY - 2016/6/14

Y1 - 2016/6/14

N2 - Cucurbit yellow stunting disorder virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a major threat to commercial cucurbit production worldwide. Here, construction of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts able to replicate in cucumber protoplasts. CYSDV RNA1 proved competent for replication; transcription of both polarities of the genomic RNA was detectable 24 h post inoculation. Hybridization of total RNA extracted from transfected protoplasts or from naturally CYSDV-infected cucurbits revealed high-level transcription of the p22 subgenomic RNA species. Replication of CYSDV RNA2 following co-transfection with RNA1 was also observed, with similar transcription kinetics. A CYSDV RNA2 cDNA clone (T3CM8Δ) comprising the 5'- and 3'-UTRs plus the 3'-terminal gene, generated a 2.8 kb RNA able to replicate to high levels in protoplasts in the presence of CYSDV RNA1. The clone T3CM8Δ will facilitate reverse genetics studies of CYSDV gene function and RNA replication determinants.

AB - Cucurbit yellow stunting disorder virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a major threat to commercial cucurbit production worldwide. Here, construction of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts able to replicate in cucumber protoplasts. CYSDV RNA1 proved competent for replication; transcription of both polarities of the genomic RNA was detectable 24 h post inoculation. Hybridization of total RNA extracted from transfected protoplasts or from naturally CYSDV-infected cucurbits revealed high-level transcription of the p22 subgenomic RNA species. Replication of CYSDV RNA2 following co-transfection with RNA1 was also observed, with similar transcription kinetics. A CYSDV RNA2 cDNA clone (T3CM8Δ) comprising the 5'- and 3'-UTRs plus the 3'-terminal gene, generated a 2.8 kb RNA able to replicate to high levels in protoplasts in the presence of CYSDV RNA1. The clone T3CM8Δ will facilitate reverse genetics studies of CYSDV gene function and RNA replication determinants.

KW - criniviruses

KW - whitefly-transmitted viruses

KW - Cucurbit yellow stunting disorder virus

KW - infectious clones

KW - protoplasts

U2 - 10.3390/v8060170

DO - 10.3390/v8060170

M3 - Article

VL - 8

SP - E170

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 6

M1 - doi: 10.3390/v8060170

ER -