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Interacting climate change factors (CO2 and temperature cycles) effects on growth, secondary metabolite gene expression and phenotypic ochratoxin A production by Aspergillus carbonarius strains on a grape-based matrix. / Cervini, C.; Verheecke-Vaessen, C.; Ferrara, M.; García-Cela, E.; Magistà, D.; Medina, A.; Gallo, A.; Magan, N.; Perrone, G.

In: Fungal Biology, 12.11.2019.

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@article{53e7dfd894a94fd5bf5999ab8ef34afd,
title = "Interacting climate change factors (CO2 and temperature cycles) effects on growth, secondary metabolite gene expression and phenotypic ochratoxin A production by Aspergillus carbonarius strains on a grape-based matrix",
abstract = "Little is known on the impact that climate change (CC) may have on Aspergillus carbonarius and Ochratoxin A (OTA) contamination of grapes, especially in the Mediterranean region where in CC scenarios temperature are expected to increase by +2–5 °C and CO2 from 400 to 800/1200 ppm. This study examined the effect of (i) current and increased temperature in the alternating 11.5 h dark/12.5 h light cycle (15–28 °C vs 18–34 °C), representative of the North Apulia area, South Italy and (ii) existing and predicted CO2 concentrations (400 vs 1000 ppm), on growth, expression of biosynthetic genes (AcOTApks, AcOTAnrps, AcOTAhal, AcOTAp450, AcOTAbZIP) and regulatory genes of Velvet complex (laeA/veA/velB, “velvet complex”) involved in OTA biosynthesis and OTA phenotypic production by three strains of A. carbonarius. The experiments made on a grape-based matrix showed that elevated CO2 resulted in a general stimulation of growth and OTA production. These results were also supported by the up-regulation of both structural and regulatory genes involved in the OTA biosynthesis. Our work has shown for the first time that elevated CO2 concentration in the Mediterranean region may result in an increased risk of OTA contamination in the wine production chain.",
keywords = "Alternating temperatures, Biosynthetic genes, Black aspergilli, Carbon dioxide, Ecophysiology, Mycotoxins",
author = "C. Cervini and C. Verheecke-Vaessen and M. Ferrara and E. Garc{\'i}a-Cela and D. Magist{\`a} and A. Medina and A. Gallo and N. Magan and G. Perrone",
note = "{\textcopyright} 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.",
year = "2019",
month = nov,
day = "12",
doi = "10.1016/j.funbio.2019.11.001",
language = "English",
journal = "Fungal Biology",
issn = "1878-6146",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Interacting climate change factors (CO2 and temperature cycles) effects on growth, secondary metabolite gene expression and phenotypic ochratoxin A production by Aspergillus carbonarius strains on a grape-based matrix

AU - Cervini, C.

AU - Verheecke-Vaessen, C.

AU - Ferrara, M.

AU - García-Cela, E.

AU - Magistà, D.

AU - Medina, A.

AU - Gallo, A.

AU - Magan, N.

AU - Perrone, G.

N1 - © 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

PY - 2019/11/12

Y1 - 2019/11/12

N2 - Little is known on the impact that climate change (CC) may have on Aspergillus carbonarius and Ochratoxin A (OTA) contamination of grapes, especially in the Mediterranean region where in CC scenarios temperature are expected to increase by +2–5 °C and CO2 from 400 to 800/1200 ppm. This study examined the effect of (i) current and increased temperature in the alternating 11.5 h dark/12.5 h light cycle (15–28 °C vs 18–34 °C), representative of the North Apulia area, South Italy and (ii) existing and predicted CO2 concentrations (400 vs 1000 ppm), on growth, expression of biosynthetic genes (AcOTApks, AcOTAnrps, AcOTAhal, AcOTAp450, AcOTAbZIP) and regulatory genes of Velvet complex (laeA/veA/velB, “velvet complex”) involved in OTA biosynthesis and OTA phenotypic production by three strains of A. carbonarius. The experiments made on a grape-based matrix showed that elevated CO2 resulted in a general stimulation of growth and OTA production. These results were also supported by the up-regulation of both structural and regulatory genes involved in the OTA biosynthesis. Our work has shown for the first time that elevated CO2 concentration in the Mediterranean region may result in an increased risk of OTA contamination in the wine production chain.

AB - Little is known on the impact that climate change (CC) may have on Aspergillus carbonarius and Ochratoxin A (OTA) contamination of grapes, especially in the Mediterranean region where in CC scenarios temperature are expected to increase by +2–5 °C and CO2 from 400 to 800/1200 ppm. This study examined the effect of (i) current and increased temperature in the alternating 11.5 h dark/12.5 h light cycle (15–28 °C vs 18–34 °C), representative of the North Apulia area, South Italy and (ii) existing and predicted CO2 concentrations (400 vs 1000 ppm), on growth, expression of biosynthetic genes (AcOTApks, AcOTAnrps, AcOTAhal, AcOTAp450, AcOTAbZIP) and regulatory genes of Velvet complex (laeA/veA/velB, “velvet complex”) involved in OTA biosynthesis and OTA phenotypic production by three strains of A. carbonarius. The experiments made on a grape-based matrix showed that elevated CO2 resulted in a general stimulation of growth and OTA production. These results were also supported by the up-regulation of both structural and regulatory genes involved in the OTA biosynthesis. Our work has shown for the first time that elevated CO2 concentration in the Mediterranean region may result in an increased risk of OTA contamination in the wine production chain.

KW - Alternating temperatures

KW - Biosynthetic genes

KW - Black aspergilli

KW - Carbon dioxide

KW - Ecophysiology

KW - Mycotoxins

UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075818625&doi=10.1016%2fj.funbio.2019.11.001&partnerID=40&md5=1c6480d1fbaa1cc6f265ebf77fb605f6

U2 - 10.1016/j.funbio.2019.11.001

DO - 10.1016/j.funbio.2019.11.001

M3 - Article

JO - Fungal Biology

JF - Fungal Biology

SN - 1878-6146

ER -