University of Hertfordshire

Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy

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Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy. / De Sousa, Karina P.; Rossi, Izadora; Abdullahi, Mahamed; Ramirez, Marcel Ivan; Stratton, Dan; Inal, Jameel Malhador.

In: Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 27.07.2022.

Research output: Contribution to journalReview articlepeer-review

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@article{43faae0083e54909b3c8956675799320,
title = "Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy",
abstract = "Abstract: Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV‐related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens. This article is categorized under: Diagnostic Tools > Biosensing Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease",
keywords = "Biosensing, Nanomedicine for Oncologic Disease, Nanomedicine for Infectious Disease, Advanced Review, Advanced Reviews, EV analysis, EV characterization, EV therapeutics, extracellular vesicles, isolation methods",
author = "{De Sousa}, {Karina P.} and Izadora Rossi and Mahamed Abdullahi and Ramirez, {Marcel Ivan} and Dan Stratton and Inal, {Jameel Malhador}",
note = "{\textcopyright} 2022 The Authors. WIREs Nanomedicine and Nanobiotechnology published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, https://creativecommons.org/licenses/by/4.0/",
year = "2022",
month = jul,
day = "27",
doi = "10.1002/wnan.1835",
language = "English",
journal = "Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology",
issn = "1939-5116",
publisher = "John Wiley & Sons, Inc.",

}

RIS

TY - JOUR

T1 - Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy

AU - De Sousa, Karina P.

AU - Rossi, Izadora

AU - Abdullahi, Mahamed

AU - Ramirez, Marcel Ivan

AU - Stratton, Dan

AU - Inal, Jameel Malhador

N1 - © 2022 The Authors. WIREs Nanomedicine and Nanobiotechnology published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, https://creativecommons.org/licenses/by/4.0/

PY - 2022/7/27

Y1 - 2022/7/27

N2 - Abstract: Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV‐related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens. This article is categorized under: Diagnostic Tools > Biosensing Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease

AB - Abstract: Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV‐related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens. This article is categorized under: Diagnostic Tools > Biosensing Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease

KW - Biosensing

KW - Nanomedicine for Oncologic Disease

KW - Nanomedicine for Infectious Disease

KW - Advanced Review

KW - Advanced Reviews

KW - EV analysis

KW - EV characterization

KW - EV therapeutics

KW - extracellular vesicles

KW - isolation methods

U2 - 10.1002/wnan.1835

DO - 10.1002/wnan.1835

M3 - Review article

JO - Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology

JF - Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology

SN - 1939-5116

M1 - e1835

ER -