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Marker-assisted selection for scald (Rhynchosporium commune L.) resistance gene(s) in barley breeding for dry areas : MAS for scald in barley. / Sayed, Haitham; Baum, Michael.

In: Journal of Plant Protection Research, Vol. 58, No. 4, 23.11.2018, p. 335-344.

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@article{f217301018b44f13bb43f9dc3d4d5809,
title = "Marker-assisted selection for scald (Rhynchosporium commune L.) resistance gene(s) in barley breeding for dry areas: MAS for scald in barley",
abstract = "Barley scald, caused by Rhynchosporium commune is one of the most prevalent diseases in barley (Hordeum vulgare L.) worldwide. The primary loss from scald is reduced yield, which can exceed 25% in dry areas. In our earlier studies, we developed a low-resolution linkage map for recombinant inbred lines of the cross Tadmor/WI2291. Quantitative trait loci (QTLs) for scald were localized on chromosomes 2H and 3H flanked by Simple Sequence Repeat (SSR) markers HVM54 and Bmac0093b on 2H and HVLTPP8, HVM62 and Bmag0006 on 3H. These chromosome 3H markers were found to be located close to theRrs1−R. commune resistance gene(s) on chromosome 3H. In this study, 10 homozygous resistant and 10 homozygous susceptible plants each from the F7 population of Tadmor/Sel160, a panel of 23 barley varieties used routinely in the International Centre for Agricultural Research in the Dry Areas (ICARDA) breeding program and three populations were used for scald resistance screening using 25 DNA markers that are located very close to scald resistance gene(s) on barley chromosomes. Only five of those markers clearly discriminated co-dominantly between resistant and susceptible plants. These markers, Ebmac0871-SSR, HVS3-SCAR, Bmag0006-SSR, reside on different arms of barley chromosome 3H. Ebmac871 is localized on the short arm of 3H and HVS3 and Bmag0006 are localized on the long arm of 3H. This result indicates that the scald resistance genes which they tag are probably close to the centromeric region of this chromosome. Scald resistance from several sources map to the proximal region of the long arm of chromosome 3H, forming the complex Rrs1 locus. The availability of highly polymorphic markers for the discrimination of breeding material would be extremely useful for barley breeders to select for the trait at the DNA level rather than relying on phenotypic expression and infection reaction.",
keywords = "barley, Marker-Assisted Selction (MAS), resistance gene, Rhynchosporium commune, scald",
author = "Haitham Sayed and Michael Baum",
year = "2018",
month = nov,
day = "23",
doi = "10.24425/jppr.2018.124642",
language = "English",
volume = "58",
pages = "335--344",
journal = "Journal of Plant Protection Research",
issn = "1427-4345",
publisher = "De Gruyter Open Ltd.",
number = "4",

}

RIS

TY - JOUR

T1 - Marker-assisted selection for scald (Rhynchosporium commune L.) resistance gene(s) in barley breeding for dry areas

T2 - MAS for scald in barley

AU - Sayed, Haitham

AU - Baum, Michael

PY - 2018/11/23

Y1 - 2018/11/23

N2 - Barley scald, caused by Rhynchosporium commune is one of the most prevalent diseases in barley (Hordeum vulgare L.) worldwide. The primary loss from scald is reduced yield, which can exceed 25% in dry areas. In our earlier studies, we developed a low-resolution linkage map for recombinant inbred lines of the cross Tadmor/WI2291. Quantitative trait loci (QTLs) for scald were localized on chromosomes 2H and 3H flanked by Simple Sequence Repeat (SSR) markers HVM54 and Bmac0093b on 2H and HVLTPP8, HVM62 and Bmag0006 on 3H. These chromosome 3H markers were found to be located close to theRrs1−R. commune resistance gene(s) on chromosome 3H. In this study, 10 homozygous resistant and 10 homozygous susceptible plants each from the F7 population of Tadmor/Sel160, a panel of 23 barley varieties used routinely in the International Centre for Agricultural Research in the Dry Areas (ICARDA) breeding program and three populations were used for scald resistance screening using 25 DNA markers that are located very close to scald resistance gene(s) on barley chromosomes. Only five of those markers clearly discriminated co-dominantly between resistant and susceptible plants. These markers, Ebmac0871-SSR, HVS3-SCAR, Bmag0006-SSR, reside on different arms of barley chromosome 3H. Ebmac871 is localized on the short arm of 3H and HVS3 and Bmag0006 are localized on the long arm of 3H. This result indicates that the scald resistance genes which they tag are probably close to the centromeric region of this chromosome. Scald resistance from several sources map to the proximal region of the long arm of chromosome 3H, forming the complex Rrs1 locus. The availability of highly polymorphic markers for the discrimination of breeding material would be extremely useful for barley breeders to select for the trait at the DNA level rather than relying on phenotypic expression and infection reaction.

AB - Barley scald, caused by Rhynchosporium commune is one of the most prevalent diseases in barley (Hordeum vulgare L.) worldwide. The primary loss from scald is reduced yield, which can exceed 25% in dry areas. In our earlier studies, we developed a low-resolution linkage map for recombinant inbred lines of the cross Tadmor/WI2291. Quantitative trait loci (QTLs) for scald were localized on chromosomes 2H and 3H flanked by Simple Sequence Repeat (SSR) markers HVM54 and Bmac0093b on 2H and HVLTPP8, HVM62 and Bmag0006 on 3H. These chromosome 3H markers were found to be located close to theRrs1−R. commune resistance gene(s) on chromosome 3H. In this study, 10 homozygous resistant and 10 homozygous susceptible plants each from the F7 population of Tadmor/Sel160, a panel of 23 barley varieties used routinely in the International Centre for Agricultural Research in the Dry Areas (ICARDA) breeding program and three populations were used for scald resistance screening using 25 DNA markers that are located very close to scald resistance gene(s) on barley chromosomes. Only five of those markers clearly discriminated co-dominantly between resistant and susceptible plants. These markers, Ebmac0871-SSR, HVS3-SCAR, Bmag0006-SSR, reside on different arms of barley chromosome 3H. Ebmac871 is localized on the short arm of 3H and HVS3 and Bmag0006 are localized on the long arm of 3H. This result indicates that the scald resistance genes which they tag are probably close to the centromeric region of this chromosome. Scald resistance from several sources map to the proximal region of the long arm of chromosome 3H, forming the complex Rrs1 locus. The availability of highly polymorphic markers for the discrimination of breeding material would be extremely useful for barley breeders to select for the trait at the DNA level rather than relying on phenotypic expression and infection reaction.

KW - barley

KW - Marker-Assisted Selction (MAS)

KW - resistance gene

KW - Rhynchosporium commune

KW - scald

U2 - 10.24425/jppr.2018.124642

DO - 10.24425/jppr.2018.124642

M3 - Article

VL - 58

SP - 335

EP - 344

JO - Journal of Plant Protection Research

JF - Journal of Plant Protection Research

SN - 1427-4345

IS - 4

ER -