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miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas. / Braoudaki, Maria; Lambrou, G I; Giannikou, K; Papadodima, S A; Lykoudi, A; Stefanaki, K; Sfakianos, G; Kolialexi, A; Tzortzatou-Stathopoulou, F; Tzetis, M; Kitsiou-Tzeli, S; Kanavakis, E.

In: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, Vol. 37, No. 7, 26.01.2016, p. 9887-97.

Research output: Contribution to journalArticle

Harvard

Braoudaki, M, Lambrou, GI, Giannikou, K, Papadodima, SA, Lykoudi, A, Stefanaki, K, Sfakianos, G, Kolialexi, A, Tzortzatou-Stathopoulou, F, Tzetis, M, Kitsiou-Tzeli, S & Kanavakis, E 2016, 'miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas', Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, vol. 37, no. 7, pp. 9887-97. https://doi.org/10.1007/s13277-016-4903-7

APA

Braoudaki, M., Lambrou, G. I., Giannikou, K., Papadodima, S. A., Lykoudi, A., Stefanaki, K., Sfakianos, G., Kolialexi, A., Tzortzatou-Stathopoulou, F., Tzetis, M., Kitsiou-Tzeli, S., & Kanavakis, E. (2016). miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 37(7), 9887-97. https://doi.org/10.1007/s13277-016-4903-7

Vancouver

Author

Braoudaki, Maria ; Lambrou, G I ; Giannikou, K ; Papadodima, S A ; Lykoudi, A ; Stefanaki, K ; Sfakianos, G ; Kolialexi, A ; Tzortzatou-Stathopoulou, F ; Tzetis, M ; Kitsiou-Tzeli, S ; Kanavakis, E. / miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas. In: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2016 ; Vol. 37, No. 7. pp. 9887-97.

Bibtex

@article{cfbd4a219e8f441f864a81c48d43be58,
title = "miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas",
abstract = "In the current setting, we attempted to verify and validate miRNA candidates relevant to pediatric primary brain tumor progression and outcome, in order to provide data regarding the identification of novel prognostic biomarkers. Overall, 26 resected brain tumors were studied from children diagnosed with pilocytic astrocytomas (PAs) (n = 19) and ependymomas (EPs) (n = 7). As controls, deceased children who underwent autopsy and were not present with any brain malignancy were used. The experimental approach included microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the expression profiles of miR-15a and miR-24-1. The multiparameter analyses were performed with MATLAB. Matching differentially expressed miRNAs were detected in both PAs and EPs, following distinct comparisons with the control cohort; however, in several cases, they exhibited tissue-specific expression profiles. On correlations between miRNA expression and EP progression or outcome, miR-15a and miR-24-1 were found upregulated in EP relapsed and EP deceased cases when compared to EP clinical remission cases and EP survivors, respectively. Taken together, following several distinct associations between miRNA expression and diverse clinical parameters, the current study repeatedly highlighted miR-15a and miR-24-1 as candidate oncogenic molecules associated with inferior prognosis in children diagnosed with ependymoma. ",
keywords = "Adolescent, Astrocytoma/genetics, Biomarkers, Tumor/genetics, Case-Control Studies, Child, Disease Progression, Ependymoma/genetics, Female, Follow-Up Studies, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Male, MicroRNAs/genetics, Neoplasm Staging, Prognosis, RNA, Messenger/genetics, ROC Curve, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction",
author = "Maria Braoudaki and Lambrou, {G I} and K Giannikou and Papadodima, {S A} and A Lykoudi and K Stefanaki and G Sfakianos and A Kolialexi and F Tzortzatou-Stathopoulou and M Tzetis and S Kitsiou-Tzeli and E Kanavakis",
year = "2016",
month = jan,
day = "26",
doi = "10.1007/s13277-016-4903-7",
language = "English",
volume = "37",
pages = "9887--97",
journal = "Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine",
issn = "1010-4283",
publisher = "SAGE Publications Inc.",
number = "7",

}

RIS

TY - JOUR

T1 - miR-15a and miR-24-1 as putative prognostic microRNA signatures for pediatric pilocytic astrocytomas and ependymomas

AU - Braoudaki, Maria

AU - Lambrou, G I

AU - Giannikou, K

AU - Papadodima, S A

AU - Lykoudi, A

AU - Stefanaki, K

AU - Sfakianos, G

AU - Kolialexi, A

AU - Tzortzatou-Stathopoulou, F

AU - Tzetis, M

AU - Kitsiou-Tzeli, S

AU - Kanavakis, E

PY - 2016/1/26

Y1 - 2016/1/26

N2 - In the current setting, we attempted to verify and validate miRNA candidates relevant to pediatric primary brain tumor progression and outcome, in order to provide data regarding the identification of novel prognostic biomarkers. Overall, 26 resected brain tumors were studied from children diagnosed with pilocytic astrocytomas (PAs) (n = 19) and ependymomas (EPs) (n = 7). As controls, deceased children who underwent autopsy and were not present with any brain malignancy were used. The experimental approach included microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the expression profiles of miR-15a and miR-24-1. The multiparameter analyses were performed with MATLAB. Matching differentially expressed miRNAs were detected in both PAs and EPs, following distinct comparisons with the control cohort; however, in several cases, they exhibited tissue-specific expression profiles. On correlations between miRNA expression and EP progression or outcome, miR-15a and miR-24-1 were found upregulated in EP relapsed and EP deceased cases when compared to EP clinical remission cases and EP survivors, respectively. Taken together, following several distinct associations between miRNA expression and diverse clinical parameters, the current study repeatedly highlighted miR-15a and miR-24-1 as candidate oncogenic molecules associated with inferior prognosis in children diagnosed with ependymoma.

AB - In the current setting, we attempted to verify and validate miRNA candidates relevant to pediatric primary brain tumor progression and outcome, in order to provide data regarding the identification of novel prognostic biomarkers. Overall, 26 resected brain tumors were studied from children diagnosed with pilocytic astrocytomas (PAs) (n = 19) and ependymomas (EPs) (n = 7). As controls, deceased children who underwent autopsy and were not present with any brain malignancy were used. The experimental approach included microarrays covering 1211 miRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the expression profiles of miR-15a and miR-24-1. The multiparameter analyses were performed with MATLAB. Matching differentially expressed miRNAs were detected in both PAs and EPs, following distinct comparisons with the control cohort; however, in several cases, they exhibited tissue-specific expression profiles. On correlations between miRNA expression and EP progression or outcome, miR-15a and miR-24-1 were found upregulated in EP relapsed and EP deceased cases when compared to EP clinical remission cases and EP survivors, respectively. Taken together, following several distinct associations between miRNA expression and diverse clinical parameters, the current study repeatedly highlighted miR-15a and miR-24-1 as candidate oncogenic molecules associated with inferior prognosis in children diagnosed with ependymoma.

KW - Adolescent

KW - Astrocytoma/genetics

KW - Biomarkers, Tumor/genetics

KW - Case-Control Studies

KW - Child

KW - Disease Progression

KW - Ependymoma/genetics

KW - Female

KW - Follow-Up Studies

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Neoplastic

KW - Humans

KW - Male

KW - MicroRNAs/genetics

KW - Neoplasm Staging

KW - Prognosis

KW - RNA, Messenger/genetics

KW - ROC Curve

KW - Real-Time Polymerase Chain Reaction

KW - Reverse Transcriptase Polymerase Chain Reaction

U2 - 10.1007/s13277-016-4903-7

DO - 10.1007/s13277-016-4903-7

M3 - Article

C2 - 26813564

VL - 37

SP - 9887

EP - 9897

JO - Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

JF - Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

SN - 1010-4283

IS - 7

ER -