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Multi-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organs. / Walsh, Claire; Holroyd, Natalie; Finnerty, Eoin; Ryan, Sean; Sweeney, Paul; Shipley, Rebecca; Walker-Samuel, Simon.

In: Elife, 07.04.2020.

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APA

Walsh, C., Holroyd, N., Finnerty, E., Ryan, S., Sweeney, P., Shipley, R., & Walker-Samuel, S. (2020). Multi-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organs. Manuscript submitted for publication. https://doi.org/10.1101/2020.04.03.023978

Vancouver

Author

Walsh, Claire ; Holroyd, Natalie ; Finnerty, Eoin ; Ryan, Sean ; Sweeney, Paul ; Shipley, Rebecca ; Walker-Samuel, Simon. / Multi-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organs. In: Elife. 2020.

Bibtex

@article{43ec51789a184728852ca29a7a166d5f,
title = "Multi-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organs",
abstract = "Three-dimensional microscopy of large biological samples (>0.5 cm3) is transforming biological research. Many existing techniques require trade-offs between image resolution and sample size, require clearing or use optical sectioning. These factors complicate the implementation of large volume 3D imaging. Here we present Multi-fluorescent High Resolution Episcopic Microscopy (MF-HREM) which allows 3D imaging of large samples without the need for clearing or optical sectioning. MF-HREM uses serial-sectioning and block-facing wide-field fluorescence, without the need for tissue clearing or optical sectioning. We detail developments in sample processing including stain penetration, resin embedding and imaging. In addition, we describe image post-processing methods needed to segment and further quantify these data. Finally, we demonstrate the wide applicability of MF-HREM by: 1) quantifying adult mouse glomeruli. 2) identifying injected cells and vascular networks in tumour xenograft models; 3) quantifying vascular networks and white matter track orientation in mouse brain.",
author = "Claire Walsh and Natalie Holroyd and Eoin Finnerty and Sean Ryan and Paul Sweeney and Rebecca Shipley and Simon Walker-Samuel",
year = "2020",
month = apr,
day = "7",
doi = "10.1101/2020.04.03.023978",
language = "English",
journal = "Elife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",

}

RIS

TY - JOUR

T1 - Multi-fluorescence high-resolution episcopic microscopy (MF-HREM) for three dimensional imaging of adult murine organs

AU - Walsh, Claire

AU - Holroyd, Natalie

AU - Finnerty, Eoin

AU - Ryan, Sean

AU - Sweeney, Paul

AU - Shipley, Rebecca

AU - Walker-Samuel, Simon

PY - 2020/4/7

Y1 - 2020/4/7

N2 - Three-dimensional microscopy of large biological samples (>0.5 cm3) is transforming biological research. Many existing techniques require trade-offs between image resolution and sample size, require clearing or use optical sectioning. These factors complicate the implementation of large volume 3D imaging. Here we present Multi-fluorescent High Resolution Episcopic Microscopy (MF-HREM) which allows 3D imaging of large samples without the need for clearing or optical sectioning. MF-HREM uses serial-sectioning and block-facing wide-field fluorescence, without the need for tissue clearing or optical sectioning. We detail developments in sample processing including stain penetration, resin embedding and imaging. In addition, we describe image post-processing methods needed to segment and further quantify these data. Finally, we demonstrate the wide applicability of MF-HREM by: 1) quantifying adult mouse glomeruli. 2) identifying injected cells and vascular networks in tumour xenograft models; 3) quantifying vascular networks and white matter track orientation in mouse brain.

AB - Three-dimensional microscopy of large biological samples (>0.5 cm3) is transforming biological research. Many existing techniques require trade-offs between image resolution and sample size, require clearing or use optical sectioning. These factors complicate the implementation of large volume 3D imaging. Here we present Multi-fluorescent High Resolution Episcopic Microscopy (MF-HREM) which allows 3D imaging of large samples without the need for clearing or optical sectioning. MF-HREM uses serial-sectioning and block-facing wide-field fluorescence, without the need for tissue clearing or optical sectioning. We detail developments in sample processing including stain penetration, resin embedding and imaging. In addition, we describe image post-processing methods needed to segment and further quantify these data. Finally, we demonstrate the wide applicability of MF-HREM by: 1) quantifying adult mouse glomeruli. 2) identifying injected cells and vascular networks in tumour xenograft models; 3) quantifying vascular networks and white matter track orientation in mouse brain.

U2 - 10.1101/2020.04.03.023978

DO - 10.1101/2020.04.03.023978

M3 - Article

JO - Elife

JF - Elife

SN - 2050-084X

ER -