University of Hertfordshire

Non-Histone Protein Methylation: Biological Significance and Bioengineering Potential

Research output: Contribution to journalArticlepeer-review


  • Roberto Di Blasi
  • Oleg Blyuss
  • John F Timms
  • Daniel Conole
  • Francesca Ceroni
  • Harry J Whitwell
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Original languageEnglish
Pages (from-to)238-250
Number of pages13
JournalACS chemical biology
Early online date7 Jan 2021
Publication statusPublished - 19 Feb 2021


Protein methylation is a key post-translational modification whose effects on gene expression have been intensively studied over the last two decades. Recently, renewed interest in non-histone protein methylation has gained momentum for its role in regulating important cellular processes and the activity of many proteins, including transcription factors, enzymes, and structural complexes. The extensive and dynamic role that protein methylation plays within the cell also highlights its potential for bioengineering applications. Indeed, while synthetic histone protein methylation has been extensively used to engineer gene expression, engineering of non-histone protein methylation has not been fully explored yet. Here, we report the latest findings, highlighting how non-histone protein methylation is fundamental for certain cellular functions and is implicated in disease, and review recent efforts in the engineering of protein methylation.


© 2021 American Chemical Society. This is the accepted manuscript version of an article which has been published in final form at

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