University of Hertfordshire

By the same authors

Documents

  • Paul L. C. Feyen
  • Bruno F. E. Matarèse
  • Laura Urbano
  • Thais F. Abelha
  • Hassan Rahmoune
  • Mark Green
  • Lea A. Dailey
  • John C. de Mello
  • Fabio Benfenati
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Original languageEnglish
Article number932877
Number of pages13
JournalFrontiers in Bioengineering and Biotechnology
Volume10
Early online date7 Jul 2022
DOIs
Publication statusPublished - 7 Jul 2022

Abstract

Conjugated polymers are increasingly exploited for biomedical applications. In this work, we explored the optical characteristics of conjugated polymers of variable chemical structures at multiple levels relevant to biological interfacing, from fluorescence yield to their influence on cellular membrane potential. We systematically compared the performance of conjugated polymer as cast thin films and as nanoparticles stabilized with amphiphilic polyethylene glycol-poly lactic acid-co-glycolic acid (PEG-PLGA). We assessed in both the dark and under illumination the stability of key optoelectronic properties in various environments, including air and biologically relevant physiological saline solutions. We found that photoreduction of oxygen correlates with nanoparticle and film degradation in physiologically relevant media. Using patch-clamp recordings in cell lines and primary neurons, we identified two broad classes of membrane potential response, which correspond to photosensitizer- and photothermal-mediated effects. Last, we introduced a metric named OED50 (optical energy for 50% depolarization), which conveys the phototoxic potency of a given agent and thereby its operational photo-safety profile.

Notes

© 2022 Feyen, Matarèse, Urbano, Abelha, Rahmoune, Green, Dailey, de Mello and Benfenati. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). https://creativecommons.org/licenses/by/4.0/

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