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Salmonella Typhimurium-specific bacteriophage SH19 and the origins of species specificity in the Vi01-like phage family. / Hooton, Steven P.T.; Timms, Andrew R.; Rowsell, Joanna; Wilson, Ray; Connerton, Ian F.

In: Virology Journal, Vol. 8, 498, 02.11.2011.

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@article{3bfd62bf7ef8490ab24dbfeef60c3b9a,
title = "Salmonella Typhimurium-specific bacteriophage SH19 and the origins of species specificity in the Vi01-like phage family",
abstract = "Background: Whole genome sequencing of bacteriophages suitable for biocontrol of pathogens in food products is a pre-requisite to any phage-based intervention procedure. Trials involving the biosanitization of Salmonella Typhimurium in the pig production environment identified one such candidate, SH19. Results: This phage was sequenced and analysis of its 157,785 bp circular dsDNA genome revealed a number of interesting features. SH19 constitutes another member of the recently-proposed Myoviridae Vi01-like family of phages, containing S. Typhi-specific Vi01 and Shigella-specific SboM-AG3. At the nucleotide level SH19 is highly similar to phage Vi01 (80-98% pairwise identity over the length of the genome), with the major differences lying in the region associated with host-range determination. Analyses of the proteins encoded within this region by SH19 revealed a cluster of three putative tail spikes. Of the three tail spikes, two have protein domains associated with the pectate lyase family of proteins (Tsp2) and P22 tail spike family (Tsp3) with the prospect that these enable Salmonella O antigen degradation. Tail spike proteins of Vi01 and SboM-AG3 are predicted to contain conserved right-handed parallel -helical structures but the internal protein domains are varied allowing different host specificities. Conclusions: The addition or exchange of tail spike protein modules is a major contributor to host range determination in the Vi01-like phage family.",
keywords = "bacteriophage genomics, biosanitization, lipopolysaccharide, Myoviridae, P22-like tail spike, pectate lyase tail spike domain, Phage biocontrol, Salmonella Typhimurium",
author = "Hooton, {Steven P.T.} and Timms, {Andrew R.} and Joanna Rowsell and Ray Wilson and Connerton, {Ian F.}",
note = "{\textcopyright} 2011 Hooton et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.",
year = "2011",
month = nov,
day = "2",
doi = "10.1186/1743-422X-8-498",
language = "English",
volume = "8",
journal = "Virology Journal",
issn = "1743-422X",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - Salmonella Typhimurium-specific bacteriophage SH19 and the origins of species specificity in the Vi01-like phage family

AU - Hooton, Steven P.T.

AU - Timms, Andrew R.

AU - Rowsell, Joanna

AU - Wilson, Ray

AU - Connerton, Ian F.

N1 - © 2011 Hooton et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PY - 2011/11/2

Y1 - 2011/11/2

N2 - Background: Whole genome sequencing of bacteriophages suitable for biocontrol of pathogens in food products is a pre-requisite to any phage-based intervention procedure. Trials involving the biosanitization of Salmonella Typhimurium in the pig production environment identified one such candidate, SH19. Results: This phage was sequenced and analysis of its 157,785 bp circular dsDNA genome revealed a number of interesting features. SH19 constitutes another member of the recently-proposed Myoviridae Vi01-like family of phages, containing S. Typhi-specific Vi01 and Shigella-specific SboM-AG3. At the nucleotide level SH19 is highly similar to phage Vi01 (80-98% pairwise identity over the length of the genome), with the major differences lying in the region associated with host-range determination. Analyses of the proteins encoded within this region by SH19 revealed a cluster of three putative tail spikes. Of the three tail spikes, two have protein domains associated with the pectate lyase family of proteins (Tsp2) and P22 tail spike family (Tsp3) with the prospect that these enable Salmonella O antigen degradation. Tail spike proteins of Vi01 and SboM-AG3 are predicted to contain conserved right-handed parallel -helical structures but the internal protein domains are varied allowing different host specificities. Conclusions: The addition or exchange of tail spike protein modules is a major contributor to host range determination in the Vi01-like phage family.

AB - Background: Whole genome sequencing of bacteriophages suitable for biocontrol of pathogens in food products is a pre-requisite to any phage-based intervention procedure. Trials involving the biosanitization of Salmonella Typhimurium in the pig production environment identified one such candidate, SH19. Results: This phage was sequenced and analysis of its 157,785 bp circular dsDNA genome revealed a number of interesting features. SH19 constitutes another member of the recently-proposed Myoviridae Vi01-like family of phages, containing S. Typhi-specific Vi01 and Shigella-specific SboM-AG3. At the nucleotide level SH19 is highly similar to phage Vi01 (80-98% pairwise identity over the length of the genome), with the major differences lying in the region associated with host-range determination. Analyses of the proteins encoded within this region by SH19 revealed a cluster of three putative tail spikes. Of the three tail spikes, two have protein domains associated with the pectate lyase family of proteins (Tsp2) and P22 tail spike family (Tsp3) with the prospect that these enable Salmonella O antigen degradation. Tail spike proteins of Vi01 and SboM-AG3 are predicted to contain conserved right-handed parallel -helical structures but the internal protein domains are varied allowing different host specificities. Conclusions: The addition or exchange of tail spike protein modules is a major contributor to host range determination in the Vi01-like phage family.

KW - bacteriophage genomics

KW - biosanitization

KW - lipopolysaccharide

KW - Myoviridae

KW - P22-like tail spike

KW - pectate lyase tail spike domain

KW - Phage biocontrol

KW - Salmonella Typhimurium

UR - http://www.scopus.com/inward/record.url?scp=80055107701&partnerID=8YFLogxK

U2 - 10.1186/1743-422X-8-498

DO - 10.1186/1743-422X-8-498

M3 - Article

C2 - 22047448

AN - SCOPUS:80055107701

VL - 8

JO - Virology Journal

JF - Virology Journal

SN - 1743-422X

M1 - 498

ER -