University of Hertfordshire

From the same journal

Standard

T-cell subpopulations αβ and γδ in cord blood of very preterm infants : The influence of intrauterine infection. / Serwatowska-Bargieł, Agata; Wa̧sik, Maria; Kornacka, Maria Katarzyna; Górska, Elzbieta; Kozarski, Robert.

In: Archivum Immunologiae et Therapiae Experimentalis, Vol. 61, No. 6, 01.12.2013, p. 495-501.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Serwatowska-Bargieł, Agata ; Wa̧sik, Maria ; Kornacka, Maria Katarzyna ; Górska, Elzbieta ; Kozarski, Robert. / T-cell subpopulations αβ and γδ in cord blood of very preterm infants : The influence of intrauterine infection. In: Archivum Immunologiae et Therapiae Experimentalis. 2013 ; Vol. 61, No. 6. pp. 495-501.

Bibtex

@article{bb66690971b9425f801f5bc56fda12de,
title = "T-cell subpopulations αβ and γδ in cord blood of very preterm infants: The influence of intrauterine infection",
abstract = "Preterm infants are very susceptible to infections. Immune response mechanisms in this group of patients and factors that influence cord blood mononuclear cell populations remain poorly understood and are considered insufficient. However, competent immune functions of the cord blood mononuclear cells are also described. The aim of this work was to evaluate the T-cell population (CD3+) with its subpopulations bearing T-cell receptor (TCR) αβ or TCR γδ in the cord blood of preterm infants born before 32 weeks of gestation by mothers with or without an intrauterine infection. Being a pilot study, it also aimed at feasibility check and assessment of an expected effect size. The cord blood samples of 46 infants age were subjected to direct immunofluorescent staining with monoclonal antibodies and then analyzed by flow cytometry. The percentage of CD3+ cells in neonates born by mothers with diagnosis of intrauterine infection was significantly lower than in neonates born by mothers without infection (p = 0.005; Mann-Whitney U test). The number of cells did not differ between groups. Infection present in the mother did not have an influence on the TCR αβ or TCR γδ subpopulations. Our study contributes to a better understanding of preterm infants' immune mechanisms, and sets the stage for further investigations.",
keywords = "CD3 cells, Gamma/delta T cells, Intrauterine infection, Preterm newborn",
author = "Agata Serwatowska-Bargie{\l} and Maria W{\c a}sik and Kornacka, {Maria Katarzyna} and Elzbieta G{\'o}rska and Robert Kozarski",
note = "Open Access: This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited",
year = "2013",
month = dec,
day = "1",
doi = "10.1007/s00005-013-0244-z",
language = "English",
volume = "61",
pages = "495--501",
journal = "Archivum Immunologiae et Therapiae Experimentalis",
issn = "0004-069X",
publisher = "Birkhauser Verlag Basel",
number = "6",

}

RIS

TY - JOUR

T1 - T-cell subpopulations αβ and γδ in cord blood of very preterm infants

T2 - The influence of intrauterine infection

AU - Serwatowska-Bargieł, Agata

AU - Wa̧sik, Maria

AU - Kornacka, Maria Katarzyna

AU - Górska, Elzbieta

AU - Kozarski, Robert

N1 - Open Access: This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Preterm infants are very susceptible to infections. Immune response mechanisms in this group of patients and factors that influence cord blood mononuclear cell populations remain poorly understood and are considered insufficient. However, competent immune functions of the cord blood mononuclear cells are also described. The aim of this work was to evaluate the T-cell population (CD3+) with its subpopulations bearing T-cell receptor (TCR) αβ or TCR γδ in the cord blood of preterm infants born before 32 weeks of gestation by mothers with or without an intrauterine infection. Being a pilot study, it also aimed at feasibility check and assessment of an expected effect size. The cord blood samples of 46 infants age were subjected to direct immunofluorescent staining with monoclonal antibodies and then analyzed by flow cytometry. The percentage of CD3+ cells in neonates born by mothers with diagnosis of intrauterine infection was significantly lower than in neonates born by mothers without infection (p = 0.005; Mann-Whitney U test). The number of cells did not differ between groups. Infection present in the mother did not have an influence on the TCR αβ or TCR γδ subpopulations. Our study contributes to a better understanding of preterm infants' immune mechanisms, and sets the stage for further investigations.

AB - Preterm infants are very susceptible to infections. Immune response mechanisms in this group of patients and factors that influence cord blood mononuclear cell populations remain poorly understood and are considered insufficient. However, competent immune functions of the cord blood mononuclear cells are also described. The aim of this work was to evaluate the T-cell population (CD3+) with its subpopulations bearing T-cell receptor (TCR) αβ or TCR γδ in the cord blood of preterm infants born before 32 weeks of gestation by mothers with or without an intrauterine infection. Being a pilot study, it also aimed at feasibility check and assessment of an expected effect size. The cord blood samples of 46 infants age were subjected to direct immunofluorescent staining with monoclonal antibodies and then analyzed by flow cytometry. The percentage of CD3+ cells in neonates born by mothers with diagnosis of intrauterine infection was significantly lower than in neonates born by mothers without infection (p = 0.005; Mann-Whitney U test). The number of cells did not differ between groups. Infection present in the mother did not have an influence on the TCR αβ or TCR γδ subpopulations. Our study contributes to a better understanding of preterm infants' immune mechanisms, and sets the stage for further investigations.

KW - CD3 cells

KW - Gamma/delta T cells

KW - Intrauterine infection

KW - Preterm newborn

U2 - 10.1007/s00005-013-0244-z

DO - 10.1007/s00005-013-0244-z

M3 - Article

C2 - 23959110

AN - SCOPUS:84890425760

VL - 61

SP - 495

EP - 501

JO - Archivum Immunologiae et Therapiae Experimentalis

JF - Archivum Immunologiae et Therapiae Experimentalis

SN - 0004-069X

IS - 6

ER -