University of Hertfordshire

By the same authors

Tendon and Ligament-Related Genes Associate with Elite Status in Rugby

Research output: Contribution to conferencePosterpeer-review

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Tendon and Ligament-Related Genes Associate with Elite Status in Rugby. / Brazier, Jon; Antrobus, M; Herbert, A; Stebbings, G; Day, S; Heffernan, S; Kilduff, L; Cullen, Tom ; Raleigh, Stuart; Erskine, R; Williams, A.

2020. Poster session presented at 25th Annual Congress of the European College of Sports Science.

Research output: Contribution to conferencePosterpeer-review

Harvard

Brazier, J, Antrobus, M, Herbert, A, Stebbings, G, Day, S, Heffernan, S, Kilduff, L, Cullen, T, Raleigh, S, Erskine, R & Williams, A 2020, 'Tendon and Ligament-Related Genes Associate with Elite Status in Rugby', 25th Annual Congress of the European College of Sports Science, 28/10/20 - 30/10/20.

APA

Brazier, J., Antrobus, M., Herbert, A., Stebbings, G., Day, S., Heffernan, S., Kilduff, L., Cullen, T., Raleigh, S., Erskine, R., & Williams, A. (2020). Tendon and Ligament-Related Genes Associate with Elite Status in Rugby. Poster session presented at 25th Annual Congress of the European College of Sports Science.

Vancouver

Brazier J, Antrobus M, Herbert A, Stebbings G, Day S, Heffernan S et al. Tendon and Ligament-Related Genes Associate with Elite Status in Rugby. 2020. Poster session presented at 25th Annual Congress of the European College of Sports Science.

Author

Brazier, Jon ; Antrobus, M ; Herbert, A ; Stebbings, G ; Day, S ; Heffernan, S ; Kilduff, L ; Cullen, Tom ; Raleigh, Stuart ; Erskine, R ; Williams, A. / Tendon and Ligament-Related Genes Associate with Elite Status in Rugby. Poster session presented at 25th Annual Congress of the European College of Sports Science.

Bibtex

@conference{8043cee5c70b43a4bad881551d696312,
title = "Tendon and Ligament-Related Genes Associate with Elite Status in Rugby",
abstract = "INTRODUCTION: Elite rugby has one of the highest reported injury incidences of any professional sport (Brooks and Kemp, 2008). Some of the most severe injuries are those affecting tendon and ligament (Brazier et al., 2019), and therefore potentially the most debilitating to a player and playing squad. Achilles tendon pathology and anterior cruciate ligament rupture are multifactorial conditions. The aetiology of these injuries are due to a number of intrinsic and extrinsic factors, with a growing body of evidence suggesting that some inter-individual variability in injury susceptibility may be due to genetic variation (September et al., 2006). Elite rugby athletes (RA) potentially have a unique ability to recover from or withstand performance limiting or career-ending soft tissue injury to achieve their elite status. Thus, it was hypothesised that RA would possess genotypes associated with reduced soft tissue injury compared to a non-athlete control population. METHODS: Participants from the RugbyGene project were elite Caucasian male RA (n = 636; mean (standard deviation) height 1.85 (0.07) m, mass 101 (12) kg, age 28 (7) yr), including 535 rugby union athletes and 104 rugby league athletes. Non-athletes (NA) were 722 Caucasian men and women (58% female; height 1.69 (0.10) m, mass 72 (14) kg, age 41 (23) yr). PCR of genomic DNA was used to determine genotypes using TaqMan probes, then groups were compared using Χ2 and odds ratio (OR) statistics, with alpha set at P<0.05. RESULTS: COLGALT1 rs8090 AA genotype was more frequent in RA (27%) compared to NA (23%) (Χ2 = 12.6, P = 0.002; OR = 1.48, 95% confidence intervals (CI) = 1.1-2.0), and NID1 rs4660148 TT genotype was more frequent in RA (10%) compared to NA (6%; Χ2 = 14.5, P = 0.001; OR = 1.6, 95% CI = 1.1-2.4). For MIR608 rs4919510, CC genotype was more frequent in RA (63%) compared to NA (56%; Χ2 = 16.4, P = 0.0003; OR = 1.7, 95% CI = 1.1-2.6). For COL3A1 rs1800255, the A-allele was more frequent in RA (26%) compared to NA (23%) due to a greater frequency of GA genotype (39%) compared to NA (33%; Χ2 = 9.0, P = 0.011; OR = 1.3, 95% CI = 1.0-1.6). There were no genotype differences between RA and NA for the COL1A1 rs1800012 variant. CONCLUSION: We provide evidence for elite RA possessing a possible protective genetic profile regarding tendon and ligament injury risk. Notably, more frequent COLGALT1 AA, NID1 TT, MIR608 CC and COL3A1 GA genotypes in RA suggest a lower genetic risk of injury could enhance career success in rugby. Future research should focus on establishing how gene variants affect the collagen and extracellular matrix structures, as this may underline appropriate interventions for a more individualised injury prevention and management plan.",
author = "Jon Brazier and M Antrobus and A Herbert and G Stebbings and S Day and S Heffernan and L Kilduff and Tom Cullen and Stuart Raleigh and R Erskine and A Williams",
year = "2020",
month = oct,
day = "28",
language = "English",
note = "25th Annual Congress of the European College of Sports Science ; Conference date: 28-10-2020 Through 30-10-2020",

}

RIS

TY - CONF

T1 - Tendon and Ligament-Related Genes Associate with Elite Status in Rugby

AU - Brazier, Jon

AU - Antrobus, M

AU - Herbert, A

AU - Stebbings, G

AU - Day, S

AU - Heffernan, S

AU - Kilduff, L

AU - Cullen, Tom

AU - Raleigh, Stuart

AU - Erskine, R

AU - Williams, A

PY - 2020/10/28

Y1 - 2020/10/28

N2 - INTRODUCTION: Elite rugby has one of the highest reported injury incidences of any professional sport (Brooks and Kemp, 2008). Some of the most severe injuries are those affecting tendon and ligament (Brazier et al., 2019), and therefore potentially the most debilitating to a player and playing squad. Achilles tendon pathology and anterior cruciate ligament rupture are multifactorial conditions. The aetiology of these injuries are due to a number of intrinsic and extrinsic factors, with a growing body of evidence suggesting that some inter-individual variability in injury susceptibility may be due to genetic variation (September et al., 2006). Elite rugby athletes (RA) potentially have a unique ability to recover from or withstand performance limiting or career-ending soft tissue injury to achieve their elite status. Thus, it was hypothesised that RA would possess genotypes associated with reduced soft tissue injury compared to a non-athlete control population. METHODS: Participants from the RugbyGene project were elite Caucasian male RA (n = 636; mean (standard deviation) height 1.85 (0.07) m, mass 101 (12) kg, age 28 (7) yr), including 535 rugby union athletes and 104 rugby league athletes. Non-athletes (NA) were 722 Caucasian men and women (58% female; height 1.69 (0.10) m, mass 72 (14) kg, age 41 (23) yr). PCR of genomic DNA was used to determine genotypes using TaqMan probes, then groups were compared using Χ2 and odds ratio (OR) statistics, with alpha set at P<0.05. RESULTS: COLGALT1 rs8090 AA genotype was more frequent in RA (27%) compared to NA (23%) (Χ2 = 12.6, P = 0.002; OR = 1.48, 95% confidence intervals (CI) = 1.1-2.0), and NID1 rs4660148 TT genotype was more frequent in RA (10%) compared to NA (6%; Χ2 = 14.5, P = 0.001; OR = 1.6, 95% CI = 1.1-2.4). For MIR608 rs4919510, CC genotype was more frequent in RA (63%) compared to NA (56%; Χ2 = 16.4, P = 0.0003; OR = 1.7, 95% CI = 1.1-2.6). For COL3A1 rs1800255, the A-allele was more frequent in RA (26%) compared to NA (23%) due to a greater frequency of GA genotype (39%) compared to NA (33%; Χ2 = 9.0, P = 0.011; OR = 1.3, 95% CI = 1.0-1.6). There were no genotype differences between RA and NA for the COL1A1 rs1800012 variant. CONCLUSION: We provide evidence for elite RA possessing a possible protective genetic profile regarding tendon and ligament injury risk. Notably, more frequent COLGALT1 AA, NID1 TT, MIR608 CC and COL3A1 GA genotypes in RA suggest a lower genetic risk of injury could enhance career success in rugby. Future research should focus on establishing how gene variants affect the collagen and extracellular matrix structures, as this may underline appropriate interventions for a more individualised injury prevention and management plan.

AB - INTRODUCTION: Elite rugby has one of the highest reported injury incidences of any professional sport (Brooks and Kemp, 2008). Some of the most severe injuries are those affecting tendon and ligament (Brazier et al., 2019), and therefore potentially the most debilitating to a player and playing squad. Achilles tendon pathology and anterior cruciate ligament rupture are multifactorial conditions. The aetiology of these injuries are due to a number of intrinsic and extrinsic factors, with a growing body of evidence suggesting that some inter-individual variability in injury susceptibility may be due to genetic variation (September et al., 2006). Elite rugby athletes (RA) potentially have a unique ability to recover from or withstand performance limiting or career-ending soft tissue injury to achieve their elite status. Thus, it was hypothesised that RA would possess genotypes associated with reduced soft tissue injury compared to a non-athlete control population. METHODS: Participants from the RugbyGene project were elite Caucasian male RA (n = 636; mean (standard deviation) height 1.85 (0.07) m, mass 101 (12) kg, age 28 (7) yr), including 535 rugby union athletes and 104 rugby league athletes. Non-athletes (NA) were 722 Caucasian men and women (58% female; height 1.69 (0.10) m, mass 72 (14) kg, age 41 (23) yr). PCR of genomic DNA was used to determine genotypes using TaqMan probes, then groups were compared using Χ2 and odds ratio (OR) statistics, with alpha set at P<0.05. RESULTS: COLGALT1 rs8090 AA genotype was more frequent in RA (27%) compared to NA (23%) (Χ2 = 12.6, P = 0.002; OR = 1.48, 95% confidence intervals (CI) = 1.1-2.0), and NID1 rs4660148 TT genotype was more frequent in RA (10%) compared to NA (6%; Χ2 = 14.5, P = 0.001; OR = 1.6, 95% CI = 1.1-2.4). For MIR608 rs4919510, CC genotype was more frequent in RA (63%) compared to NA (56%; Χ2 = 16.4, P = 0.0003; OR = 1.7, 95% CI = 1.1-2.6). For COL3A1 rs1800255, the A-allele was more frequent in RA (26%) compared to NA (23%) due to a greater frequency of GA genotype (39%) compared to NA (33%; Χ2 = 9.0, P = 0.011; OR = 1.3, 95% CI = 1.0-1.6). There were no genotype differences between RA and NA for the COL1A1 rs1800012 variant. CONCLUSION: We provide evidence for elite RA possessing a possible protective genetic profile regarding tendon and ligament injury risk. Notably, more frequent COLGALT1 AA, NID1 TT, MIR608 CC and COL3A1 GA genotypes in RA suggest a lower genetic risk of injury could enhance career success in rugby. Future research should focus on establishing how gene variants affect the collagen and extracellular matrix structures, as this may underline appropriate interventions for a more individualised injury prevention and management plan.

M3 - Poster

T2 - 25th Annual Congress of the European College of Sports Science

Y2 - 28 October 2020 through 30 October 2020

ER -