University of Hertfordshire

From the same journal

The phagocytic capacity of neurones

Research output: Contribution to journalArticlepeer-review

  • Samantha Bowen
  • Davidson D. Ateh
  • Katrin Deinhardt
  • Margaret M. Bird
  • Karen M. Price
  • Cathy S. Baker
  • Joanna C. Robson
  • Michael Swash
  • Wassim Shamsuddin
  • Shalini Kawar
  • Tariq El-Tawil
  • Jesper Roos
  • Andrew Hoyle
  • Carole D. Nickols
  • Charles H. Knowles
  • Anthony H. Pullen
  • Phillip J. Luthert
  • Roy O. Weller
  • Majid Hafezparast
  • Robin J. M. Franklin
  • And 6 others
  • Tamas Revesz
  • Rosalind H. M. King
  • Otto Berninghausen
  • Elizabeth M. C. Fisher
  • Giampietro Schiavo
  • Joanne E. Martin
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Original languageEnglish
Pages (from-to)2947-55
Number of pages9
JournalEuropean Journal of Neuroscience
Volume25
Issue10
DOIs
Publication statusPublished - May 2007

Abstract

Phagocytosis is defined as the ingestion of particulates over 0.5 microm in diameter and is associated with cells of the immune system such as macrophages or monocytes. Neurones are not generally recognized to be phagocytic. Using light, confocal, time-lapse and electron microscopy, we carried out a wide range of in-vitro and in-vivo experiments to examine the phagocytic capacity of different neuronal cell types. We demonstrated phagocytosis of material by neurones, including cell debris and synthetic particles up to 2.8 microm in diameter. We showed phagocytosis in different neuronal types, and demonstrated that debris can be transported from neurite extremities to cell bodies and persist within neurones. Flow cytometry analysis demonstrated the lack of certain complement receptors on neurones but the presence of others, including integrin receptors known to mediate macrophage phagocytosis, indicating that a restricted set of phagocytosis receptors may mediate this process. Neuronal phagocytosis occurs in vitro and in vivo, and we propose that this is a more widespread and significant process than previously recognized. Neuronal phagocytosis may explain certain inclusions in neurones during disease, cell-to-cell spread of disease, neuronal death during disease progression and provide a potential mechanism for therapeutic intervention through the delivery of particulate drug carriers.

ID: 1995539