University of Hertfordshire

  • Xavier Portell
  • Carol Verheecke-Vaessen
  • Rosa torrelles-Rafales
  • Angel Medina
  • Wilfred Otten
  • Naresh Magan
  • Esther Garcia-Cela
View graph of relations
Original languageEnglish
Article number1170
Number of pages18
JournalMicroorganisms
Volume8
Issue8
DOIs
Publication statusPublished - 1 Aug 2020

Abstract

Fusarium causes significant post-harvest quality losses and mycotoxin contamination instored wheat but the colonisation dynamics of the grain and how this may be affected by the initialinoculum position in the grain mass is poorly understood. This study examined the 3D growthkinetics and mycotoxin production (deoxynivalenol and zearalenone) by F. graminearum duringhyphal colonisation from different initial inoculum positions in wheat microcosms (top-centre,bottom-centre, and bottom-side) maintained at two water activities (aw; 0.95 and 0.97). Clear jarswere used to visually follow the colonisation dynamics. Fungal respiration and associated drymatter loss (DML) and ergosterol were also quantified. Colonisation dynamics was shown to beaffected by the inoculation position. At the end of the colonisation process, fungal respiration andDML were driven by the inoculation position, and the latter also by the prevailing aw. Fungalbiomass (ergosterol) was mainly affected by the aw. The initial inoculum position did not affect therelative mycotoxin production. There was a positive correlation between respiration and ergosterol,and between mycotoxin production and colonisation indicators. We suggest that spatially explicitpredictive models can be used to better understand the colonisation patterns and mycotoxincontamination of stored cereal commodities and to aid more effective post-harvest management.

Notes

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

ID: 22422722