University of Hertfordshire

From the same journal

By the same authors

Standard

Unveiling the effect of interacting forecasted abiotic factors on growth and aflatoxin B1 production kinetics by Aspergillus flavus. / Garcia-Cela, Esther; Verheecke-Vaessen, Carol; Gutierrez-Pozo, Maria; Kiaitsi, Elisavet; Gasperini, Alessandra M.; Magan, Naresh; Medina, Angel.

In: Fungal Biology, 30.05.2020.

Research output: Contribution to journalArticle

Harvard

APA

Vancouver

Author

Garcia-Cela, Esther ; Verheecke-Vaessen, Carol ; Gutierrez-Pozo, Maria ; Kiaitsi, Elisavet ; Gasperini, Alessandra M. ; Magan, Naresh ; Medina, Angel. / Unveiling the effect of interacting forecasted abiotic factors on growth and aflatoxin B1 production kinetics by Aspergillus flavus. In: Fungal Biology. 2020.

Bibtex

@article{f868a3c8f4c74c5ba77d70bfcc3fef74,
title = "Unveiling the effect of interacting forecasted abiotic factors on growth and aflatoxin B1 production kinetics by Aspergillus flavus",
abstract = "The aim was to decipher the temporal impact of key interacting climate change (CC) abiotic factors of temperature (30 vs 37 °C), water activity (aw; 0.985 vs 0.930) and CO2 exposure (400 vs 1000 ppm) on (a) growth of Aspergillus flavus and effects on (b) gene expression of a structural (aflD) and key regulatory gene (aflR) involved in aflatoxin B1 (AFB1) biosynthesis and (c) AFB1 production on a yeast extract sucrose medium over a period of 10 days. A. flavus grew and produced AFB1 very early with toxin detected after only 48 h. Both growth and toxin production were significantly impacted by the interacting abiotic factors. The relative expression of the aflD gene was significantly influenced by temperature; aflR gene expression was mainly modulated by time. However, no clear relationship was observed for both genes with AFB1 production over the experimental time frame. The optimum temperature for AFB1 production was 30 °C. Maximum AFB1 production occurred between days 4–8. Exposure to higher CO2 conditions simulating forecasted CC conditions resulted in the amount of AFB1 produced in elevated temperature (37 °C) being higher than with the optimum temperature (30 °C) showing a potential for increased risk for human/animal health due to higher accumulation of this toxin.",
keywords = "aflD, aflR, Elevated CO, Mycotoxins, Toxin gene expression, Water activity",
author = "Esther Garcia-Cela and Carol Verheecke-Vaessen and Maria Gutierrez-Pozo and Elisavet Kiaitsi and Gasperini, {Alessandra M.} and Naresh Magan and Angel Medina",
note = "{\textcopyright} 2020 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/.",
year = "2020",
month = may,
day = "30",
doi = "10.1016/j.funbio.2020.05.003",
language = "English",
journal = "Fungal Biology",
issn = "1878-6146",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Unveiling the effect of interacting forecasted abiotic factors on growth and aflatoxin B1 production kinetics by Aspergillus flavus

AU - Garcia-Cela, Esther

AU - Verheecke-Vaessen, Carol

AU - Gutierrez-Pozo, Maria

AU - Kiaitsi, Elisavet

AU - Gasperini, Alessandra M.

AU - Magan, Naresh

AU - Medina, Angel

N1 - © 2020 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/.

PY - 2020/5/30

Y1 - 2020/5/30

N2 - The aim was to decipher the temporal impact of key interacting climate change (CC) abiotic factors of temperature (30 vs 37 °C), water activity (aw; 0.985 vs 0.930) and CO2 exposure (400 vs 1000 ppm) on (a) growth of Aspergillus flavus and effects on (b) gene expression of a structural (aflD) and key regulatory gene (aflR) involved in aflatoxin B1 (AFB1) biosynthesis and (c) AFB1 production on a yeast extract sucrose medium over a period of 10 days. A. flavus grew and produced AFB1 very early with toxin detected after only 48 h. Both growth and toxin production were significantly impacted by the interacting abiotic factors. The relative expression of the aflD gene was significantly influenced by temperature; aflR gene expression was mainly modulated by time. However, no clear relationship was observed for both genes with AFB1 production over the experimental time frame. The optimum temperature for AFB1 production was 30 °C. Maximum AFB1 production occurred between days 4–8. Exposure to higher CO2 conditions simulating forecasted CC conditions resulted in the amount of AFB1 produced in elevated temperature (37 °C) being higher than with the optimum temperature (30 °C) showing a potential for increased risk for human/animal health due to higher accumulation of this toxin.

AB - The aim was to decipher the temporal impact of key interacting climate change (CC) abiotic factors of temperature (30 vs 37 °C), water activity (aw; 0.985 vs 0.930) and CO2 exposure (400 vs 1000 ppm) on (a) growth of Aspergillus flavus and effects on (b) gene expression of a structural (aflD) and key regulatory gene (aflR) involved in aflatoxin B1 (AFB1) biosynthesis and (c) AFB1 production on a yeast extract sucrose medium over a period of 10 days. A. flavus grew and produced AFB1 very early with toxin detected after only 48 h. Both growth and toxin production were significantly impacted by the interacting abiotic factors. The relative expression of the aflD gene was significantly influenced by temperature; aflR gene expression was mainly modulated by time. However, no clear relationship was observed for both genes with AFB1 production over the experimental time frame. The optimum temperature for AFB1 production was 30 °C. Maximum AFB1 production occurred between days 4–8. Exposure to higher CO2 conditions simulating forecasted CC conditions resulted in the amount of AFB1 produced in elevated temperature (37 °C) being higher than with the optimum temperature (30 °C) showing a potential for increased risk for human/animal health due to higher accumulation of this toxin.

KW - aflD

KW - aflR

KW - Elevated CO

KW - Mycotoxins

KW - Toxin gene expression

KW - Water activity

UR - http://www.scopus.com/inward/record.url?scp=85086162027&partnerID=8YFLogxK

U2 - 10.1016/j.funbio.2020.05.003

DO - 10.1016/j.funbio.2020.05.003

M3 - Article

AN - SCOPUS:85086162027

JO - Fungal Biology

JF - Fungal Biology

SN - 1878-6146

ER -