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Whole-Genome Analysis of 60 G Protein-Coupled Receptors in Caenorhabditis elegans by Gene Knockout with RNAi. / Keating, Christopher; Kriek, Neline; Daniels, Margaret; Ashcroft, Neville R.; Hopper, Neil A.; Siney, Elodie J.; Holden-Dye, Lindy; Burke, Julian F.

In: Current Biology, Vol. 13, No. 19, 09.2003, p. 1715-1720.

Research output: Contribution to journalArticlepeer-review

Harvard

Keating, C, Kriek, N, Daniels, M, Ashcroft, NR, Hopper, NA, Siney, EJ, Holden-Dye, L & Burke, JF 2003, 'Whole-Genome Analysis of 60 G Protein-Coupled Receptors in Caenorhabditis elegans by Gene Knockout with RNAi', Current Biology, vol. 13, no. 19, pp. 1715-1720. https://doi.org/10.1016/j.cub.2003.09.003

APA

Keating, C., Kriek, N., Daniels, M., Ashcroft, N. R., Hopper, N. A., Siney, E. J., Holden-Dye, L., & Burke, J. F. (2003). Whole-Genome Analysis of 60 G Protein-Coupled Receptors in Caenorhabditis elegans by Gene Knockout with RNAi. Current Biology, 13(19), 1715-1720. https://doi.org/10.1016/j.cub.2003.09.003

Vancouver

Author

Keating, Christopher ; Kriek, Neline ; Daniels, Margaret ; Ashcroft, Neville R. ; Hopper, Neil A. ; Siney, Elodie J. ; Holden-Dye, Lindy ; Burke, Julian F. / Whole-Genome Analysis of 60 G Protein-Coupled Receptors in Caenorhabditis elegans by Gene Knockout with RNAi. In: Current Biology. 2003 ; Vol. 13, No. 19. pp. 1715-1720.

Bibtex

@article{058b4d0d5760430885b5d2a0f85308c3,
title = "Whole-Genome Analysis of 60 G Protein-Coupled Receptors in Caenorhabditis elegans by Gene Knockout with RNAi",
abstract = "G protein-coupled receptors (GPCRs) are the largest family of genes in animal genomes and represent more than 2% of genes in humans and C. elegans. These evolutionarily conserved seven-transmembrane proteins transduce a diverse range of signals. In view of their pivotal role in cell signaling, it is perhaps surprising that decades of genetic analysis in C. elegans, and recent genome-wide RNAi screens, have identified very few GPCR mutants [1,2]. Therefore, we screened all GPCRs predicted to bind either small-molecule neurotransmitters or neuropeptides by using RNAi and quantitative behavioral assays. This shows that C16D6.2, C25G6.5, C26F1.6, F35G8.1, F41E7.3, and F59C12.2 are likely to be involved in reproduction, whereas C15B12.5, C10C6.2, C24A8.4, F15A8.5, F59D12.1, T02E9.1, and T05A1.1 have a role in locomotion. Gene deletions for F35G8.1 and T05A1.1 resulted in the same phenotype as that seen with RNAi. As some GPCRs may be resistant to RNAi, or may result in abnormalities not screened for here, the actual proportion of nonredundant receptors with an assayable function is probably greater. Strikingly, most phenotypes were observed for NPY-like receptors that may bind neuropeptides. This is consistent with the known actions of neuropeptides on the body wall muscle and reproductive tract in nematodes",
author = "Christopher Keating and Neline Kriek and Margaret Daniels and Ashcroft, {Neville R.} and Hopper, {Neil A.} and Siney, {Elodie J.} and Lindy Holden-Dye and Burke, {Julian F.}",
year = "2003",
month = sep,
doi = "10.1016/j.cub.2003.09.003",
language = "English",
volume = "13",
pages = "1715--1720",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "19",

}

RIS

TY - JOUR

T1 - Whole-Genome Analysis of 60 G Protein-Coupled Receptors in Caenorhabditis elegans by Gene Knockout with RNAi

AU - Keating, Christopher

AU - Kriek, Neline

AU - Daniels, Margaret

AU - Ashcroft, Neville R.

AU - Hopper, Neil A.

AU - Siney, Elodie J.

AU - Holden-Dye, Lindy

AU - Burke, Julian F.

PY - 2003/9

Y1 - 2003/9

N2 - G protein-coupled receptors (GPCRs) are the largest family of genes in animal genomes and represent more than 2% of genes in humans and C. elegans. These evolutionarily conserved seven-transmembrane proteins transduce a diverse range of signals. In view of their pivotal role in cell signaling, it is perhaps surprising that decades of genetic analysis in C. elegans, and recent genome-wide RNAi screens, have identified very few GPCR mutants [1,2]. Therefore, we screened all GPCRs predicted to bind either small-molecule neurotransmitters or neuropeptides by using RNAi and quantitative behavioral assays. This shows that C16D6.2, C25G6.5, C26F1.6, F35G8.1, F41E7.3, and F59C12.2 are likely to be involved in reproduction, whereas C15B12.5, C10C6.2, C24A8.4, F15A8.5, F59D12.1, T02E9.1, and T05A1.1 have a role in locomotion. Gene deletions for F35G8.1 and T05A1.1 resulted in the same phenotype as that seen with RNAi. As some GPCRs may be resistant to RNAi, or may result in abnormalities not screened for here, the actual proportion of nonredundant receptors with an assayable function is probably greater. Strikingly, most phenotypes were observed for NPY-like receptors that may bind neuropeptides. This is consistent with the known actions of neuropeptides on the body wall muscle and reproductive tract in nematodes

AB - G protein-coupled receptors (GPCRs) are the largest family of genes in animal genomes and represent more than 2% of genes in humans and C. elegans. These evolutionarily conserved seven-transmembrane proteins transduce a diverse range of signals. In view of their pivotal role in cell signaling, it is perhaps surprising that decades of genetic analysis in C. elegans, and recent genome-wide RNAi screens, have identified very few GPCR mutants [1,2]. Therefore, we screened all GPCRs predicted to bind either small-molecule neurotransmitters or neuropeptides by using RNAi and quantitative behavioral assays. This shows that C16D6.2, C25G6.5, C26F1.6, F35G8.1, F41E7.3, and F59C12.2 are likely to be involved in reproduction, whereas C15B12.5, C10C6.2, C24A8.4, F15A8.5, F59D12.1, T02E9.1, and T05A1.1 have a role in locomotion. Gene deletions for F35G8.1 and T05A1.1 resulted in the same phenotype as that seen with RNAi. As some GPCRs may be resistant to RNAi, or may result in abnormalities not screened for here, the actual proportion of nonredundant receptors with an assayable function is probably greater. Strikingly, most phenotypes were observed for NPY-like receptors that may bind neuropeptides. This is consistent with the known actions of neuropeptides on the body wall muscle and reproductive tract in nematodes

U2 - 10.1016/j.cub.2003.09.003

DO - 10.1016/j.cub.2003.09.003

M3 - Article

VL - 13

SP - 1715

EP - 1720

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 19

ER -